Methylation and Related Modifications Analysis Service
MtoZ Biolabs provides Methylation and Related Modifications Analysis Service to comprehensively characterize protein methylation and its biological significance. Leveraging advanced LC-MS/MS technology, optimized enrichment strategies, and quantitative proteomics workflows, our platform enables precise identification and quantification of methylated proteins across diverse sample types.
Through label-free, TMT, and iTRAQ-based quantification, our Methylation and Related Modifications Analysis Service helps researchers systematically compare methylation levels across biological conditions, uncovering subtle regulatory differences and functional insights that would otherwise be missed using conventional approaches.
What Is Methylation and Related Modifications?
Protein methylation is a pivotal post-translational modification (PTM) that regulates chromatin structure, gene expression, signal transduction, and protein–protein interactions. It typically occurs on lysine (Lys) or arginine (Arg) residues through the addition of one or more methyl groups catalyzed by methyltransferases.
Depending on the number and pattern of methyl groups added, methylation can take several forms such as monomethylation, dimethylation, and trimethylation, each exerting distinct structural and regulatory consequences. Moreover, dimethylation can exist as asymmetric or symmetric, influencing separate cellular pathways including RNA processing, immune response, and chromatin remodeling.
Methylation often acts in coordination with other PTMs such as acetylation, phosphorylation, and ubiquitination, forming complex regulatory networks that finely tune protein function and gene activity.
Services at MtoZ Biolabs
1. Targeted Methylation and Related Modifications Analysis
MtoZ Biolabs provides targeted analysis of methylation and related modifications on client-specified proteins. This service delivers precise site-level identification and quantitative assessment of individual targets, covering mono, di, and trimethylation as well as associated regulatory variants. It is suitable for focused projects that require detailed evaluation of selected proteins under defined biological or experimental settings.
2. Methylation and Related Modifications Proteomics
For broader investigations, MtoZ Biolabs offers proteome-wide analysis of methylation and related modifications in complex biological samples. This service supports system-level studies by characterizing global modification patterns, enabling comparative assessments across tissues, cell types, conditions, or disease states, and facilitating the discovery of modification signatures with functional or biomarker relevance.
To comprehensively characterize these events, MtoZ Biolabs provides specialized analytical services for lysine methylation and its related subtypes, enabling high-confidence identification of modification sites and quantitative assessment of their biological relevance.
Analysis Workflow
Figure 1. Methylation and Related Modifications Analysis Workflow
Sample Submission Suggestions
Note: Samples should be frozen at -80°C and shipped on dry ice. For complex or low-abundance samples, please consult our technical experts for pre-assessment and optimal preparation recommendations.
Applications
The Methylation and Related Modifications Analysis Service provides valuable insights across multiple research disciplines.
● Epigenetics: Investigating methylation-dependent chromatin regulation and transcriptional control.
● Oncology: Profiling methylation signatures associated with tumor progression, metastasis, and drug resistance.
● Neuroscience: Studying methylation-related mechanisms in neuronal development and neurodegenerative disorders.
● Immunology: Understanding the role of methyltransferases in immune signaling and inflammatory regulation.
● Drug Development: Supporting discovery and validation of small-molecule inhibitors targeting methyltransferases and demethylases.
Why Choose MtoZ Biolabs?
✅ Comprehensive PTM Expertise: Specialized methylation proteomics platform integrating state-of-the-art LC-MS/MS and bioinformatics.
✅ Accurate Quantification: Multiple quantification options to meet diverse experimental needs.
✅ High Data Quality Assurance: Multi-level QC and AI-driven data processing for maximum reliability.
✅ Flexible Sample Compatibility: Applicable to cell lines, tissues, biofluids, and purified proteins.
✅ Customized Research Solutions: Tailored experimental design and interpretation aligned with client objectives.
✅ One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
What Could be Included in the Report?
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment
4. Data Analysis, Preprocessing, and Estimation
5. Bioinformatics Analysis
6. Raw Data Files
Methylation and Related Modifications Analysis Service at MtoZ Biolabs delivers dependable, high-resolution insights into protein methylation and its regulatory roles in biological systems. Through rigorous analytical workflows and precise data interpretation, we help researchers uncover molecular mechanisms that drive gene regulation and cellular function.
Our scientific team is committed to providing reliable analytical support and clear biological interpretation. To learn how our expertise can advance your research, contact MtoZ Biolabs for professional consultation.
FAQ
Q1: How does MtoZ Biolabs differentiate between mono, di, and trimethylation states?
We apply high-resolution LC-MS/MS combined with optimized fragmentation and accurate mass measurement. Each methylation level corresponds to a distinct mass shift, enabling confident discrimination and localization of modification sites.
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