Methacrylation (Kmea) Analysis Service

Methacrylation (Kmea) is an emerging post-translational modification involving the covalent attachment of methacryl groups to lysine residues. This modification is increasingly recognized for its regulatory roles in protein structure, enzymatic function, and cellular signaling.

To advance methacrylation research, MtoZ Biolabs provides a comprehensive Methacrylation (Kmea) Analysis Service covering both target protein analysis and proteome-wide profiling. Leveraging high-resolution LC–MS/MS platforms, optimized enrichment techniques, and data-driven bioinformatics, we deliver precise identification, quantification, and interpretation of Kmea modifications across diverse biological samples.

1. Target Protein Methacrylation Analysis

This service focuses on the precise detection and characterization of methacrylation on specific proteins of interest. Through selective antibody-based enrichment and high-resolution mass spectrometry, MtoZ Biolabs identifies modification sites and quantifies methacrylation levels with high confidence. 

2. Methacrylation Proteomics

For large-scale studies, our methacrylation proteomics service enables systematic profiling of Kmea-modified proteins across the entire proteome. Utilizing advanced LC–MS/MS workflows integrated with quantitative strategies such as TMT or label-free analysis, we comprehensively map methacrylation sites, compare modification patterns across conditions, and perform downstream functional annotation including pathway and network analyses. This system-wide perspective helps reveal the broader biological significance of methacrylation and its role in complex regulatory circuits.

What is Methacrylation?

Protein methacrylation involves the covalent attachment of a methacryloyl group to lysine side chains, a process mediated by enzymatic transfer reactions that link cellular metabolism to protein regulation. Through altering the chemical environment of lysine residues, methacrylation has been associated with multiple biological processes, including transcriptional regulation, chromatin organization, protein interaction networks, and cellular signaling pathways. A unique feature of methacrylation is its close relationship to lysine crotonylation (Kcr). Both modifications generate an identical mass shift (+68.023 Da) in mass spectrometry, yet subtle structural differences between the two result in divergent biological functions and regulatory mechanisms. This distinction highlights the importance of precise analytical methods to accurately differentiate Kmea from other acylation types and to uncover its unique contributions to physiology and disease.

Figure 1. Lysine Methacrylation and Crotonylation

Analysis Workflow

Sample Submission Suggestions

For other sample types, please contact us for customized guidance.

• Preservation: Snap-freeze samples in liquid nitrogen and store at –80°C.

• Shipping: Transport samples on dry ice to maintain stability.

• Replicates: Provide biological replicates to support statistical analysis.

Service Advantages

✅ Advanced Platforms

Cutting-edge Methacrylation (Kmea) Analysis Service platform delivers high sensitivity and resolution.

✅ Professional Expertise

Experienced researchers with strong backgrounds in proteomics and biology.

✅ Comprehensive Workflow

End-to-end solution from enrichment to bioinformatics interpretation.

✅ Broad Sample Compatibility

Suitable for cells, tissues, plasma, serum, and purified proteins.

✅ Stable and Reproducible

Standardized protocols ensure reproducibility and consistency.

Applications

The analysis of methacrylation modifications provides insights across multiple research fields:

1. Protein Structure and Function

Revealing how Kmea alters protein folding, stability, and enzymatic activity.

2. Metabolic Regulation

Linking methacrylation to metabolic intermediates and their influence on protein networks.

3. Epigenetics and Gene Regulation

Investigating whether methacrylation affects chromatin remodeling and transcriptional control.

4. Disease Mechanisms

Exploring potential roles of Kmea in cancer, metabolic disorders, and neurodegenerative diseases.

5. Biomarker Discovery

Identifying methacrylation signatures as indicators of physiological and pathological states.

6. Drug Development

Assessing how therapeutic interventions may influence Kmea dynamics in target proteins.

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)

4. Data Analysis, Preprocessing, and Estimation (project-dependent)

5. Bioinformatics Analysis

6. Raw Data Files

Methacrylation (Kmea) represents a promising but underexplored post-translational modification with potential implications for protein regulation, metabolism, and disease biology. Accurate characterization of this modification requires sensitive detection, selective enrichment, and rigorous data analysis. Our comprehensive workflows and professional support ensure reliable, reproducible, and high-confidence results that can accelerate your research and facilitate novel discoveries. Free project evaluation, welcome to learn more details.