Ubiquitin-like Modifications Analysis Service
Ubiquitin-like Modifications (UBLs) are a class of protein post-translational modifications (PTMs) that are highly similar to ubiquitination mechanisms. Through a cascade of E1 activating enzymes, E2 conjugating enzymes, and E3 ligases, small ubiquitin-like proteins (such as SUMO, NEDD8 or Pup) are covalently attached to the lysine residues of target proteins, thereby regulating protein stability, localization, activity, and their roles in cellular signaling networks.
The analysis of UBLs has important significance in life sciences and biomedical research. It not only helps researchers uncover the regulatory mechanisms in key pathways such as DNA repair, cell cycle, and immune response, but also provides new insights for disease research and drug development. Since UBL abnormalities are closely associated with cancer, inflammation, and neurodegenerative diseases, their detection and analysis have become essential tools for studying molecular mechanisms and identifying novel therapeutic targets.
Figure 2. Identification of Ubiquitin-like Modifications Proteins
Mass spectrometry is the core approach for analyzing Ubiquitin-like Modifications, enabling precise identification and quantification of modified proteins and sites. MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics,metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. With advanced mass spectrometry platforms and a well-established experimental system, MtoZ Biolabs offers a high-quality ubiquitin-like modifications analysis service that provides reliable data support for researchers and pharmaceutical development teams to accelerate scientific progress.
Services at MtoZ Biolabs
MtoZ Biolabs provides a comprehensive ubiquitin-like modifications analysis service that includes precise identification of modified proteins and sites, quantitative comparison of modification levels under different conditions, and functional annotation and pathway insights. Our services include, but are not limited to:
1. Target Protein Ubiquitin-Like Modifications Analysis
MtoZ Biolabs Target Protein Ubiquitin-Like Modifications Analysis enables the detection of various ubiquitin-like modifications on specific proteins and provides key information such as modification occurrence, modification sites, modification types, and abundance changes. By applying multiple enrichment strategies, including antibody enrichment, chemical capture, and affinity-based methods, we enhance the detection of modified peptides and combine these approaches with high-resolution LC-MS/MS using CID, HCD, ETD, and EThcD fragmentation to accurately identify and quantify modifications such as SUMOylation and NEDDylation. This service supports clients in obtaining comprehensive information on the modification status of their target proteins.
2. Ubiquitin-Like Modifications Proteomics Analysis
MtoZ Biolabs Ubiquitin-Like Modifications Proteomics Analysis is designed for large-scale studies and enables systematic identification and quantification of ubiquitin-like modifications across the entire proteome. This service provides modified protein lists, site information, abundance matrices, and modification changes under different experimental conditions. Through antibody enrichment and tag-based capture strategies combined with high-throughput LC-MS/MS and quantitative approaches such as TMT or Label-free, we enable cross-sample quantification suitable for constructing modification profiles, comparing differences between treatment groups, and monitoring dynamic modification changes.
Service Advantages
1. Advanced Analysis Platform
MtoZ Biolabs is equipped with high-resolution mass spectrometry systems such as Thermo Fisher Orbitrap Fusion Lumos and Q Exactive HF, combined with Nano-LC separation platforms, providing reliable support for site identification and quantitative analysis of Ubiquitin-like Modifications.
2. Optimization for Low-Abundance Modifications
UBL-modified proteins are often low in abundance. We provide dedicated enrichment strategies to ensure low-abundance modifications are not missed, improving detection depth.
3. Integration of Qualitative and Quantitative Analysis
Our ubiquitin-like modifications analysis service not only identifies modified proteins and modification sites but also quantitatively analyzes their abundance changes, revealing dynamic regulatory roles under different conditions.
4. Flexible Experimental Design
We can adjust workflows according to customer research goals and sample types, adapting to both exploratory studies and large-scale systematic research.
5. Comprehensive Data Delivery
We deliver detailed experimental reports, raw mass spectrometry files, and analysis results, combined with functional annotation, to help researchers accelerate their studies.
Applications
✔️ Cancer Research: Reveal abnormal patterns of UBL modifications in tumor development.
✔️ DNA Repair Research: Explore the role of SUMOylation and Neddylation in maintaining genome stability.
✔️ Immunity and Infection Research: Study the functions of ISGylation and FAT10ylation in antiviral defense and immune regulation.
✔️ Protein Homeostasis Research: Analyze the significance of Ufmylation and Pupylation in protein degradation and homeostasis regulation.
✔️ Drug Development: Identify potential therapeutic targets related to UBLs and provide data support for new drug development.
With high-resolution mass spectrometry platforms and well-established experimental workflows, MtoZ Biolabs provides ubiquitin-like modifications analysis service covering multiple modification types, helping researchers comprehensively analyze modified proteins, sites, and dynamic changes. We are committed to providing high-quality, reproducible, and interpretable data support to promote efficient progress in scientific projects.
If you are conducting research related to UBLs, please contact us and let MtoZ Biolabs be your trusted partner.