Lysine Methylation Analysis Service
MtoZ Biolabs provides Lysine Methylation Analysis Service to enable precise identification, mapping, and quantification of lysine methylation across diverse protein samples. With advanced Orbitrap Fusion Lumos and Q Exactive HF mass spectrometry platforms, specialized enrichment techniques, and bioinformatics-driven interpretation, our service delivers reproducible, publication-ready datasets that reveal the functional impact of lysine methylation. By addressing the challenges of low-abundance modifications and complex peptide environments, MtoZ Biolabs empowers researchers to investigate lysine methylation in health and disease with confidence.
What is Lysine Methylation?
Lysine methylation is a post-translational modification in which one, two, or three methyl groups are added to the ε-amino group of lysine residues. It is catalyzed by lysine methyltransferases (KMTs) and removed by lysine demethylases (KDMs). This modification occurs on histone and non-histone proteins, regulating gene expression, chromatin structure, protein stability, and signal transduction. Depending on its methylation state, including mono, di, or tri methylation, lysine methylation can exert distinct biological outcomes. Dysregulated lysine methylation has been implicated in cancer, immune disorders, and neurological diseases, making it a critical focus in epigenetics and proteomics research.
Figure 1. Lysine Methylation
Services at MtoZ Biolabs
1. Targeted Protein Lysine Methylation Analysis
MtoZ Biolabs offers targeted lysine methylation analysis for client-designated proteins, providing accurate site-level identification and quantitative assessment of methylation on specific targets. This service is suitable for studies requiring focused evaluation of known proteins, such as target validation, pathway-specific research, and modification monitoring under defined experimental conditions.
2. Lysine Methylation Proteomics
For system-wide investigations, MtoZ Biolabs provides lysine methylation proteomics to profile methylation events across all proteins in complex biological samples. This global approach supports discovery research, comparative analyses across biological states, and the identification of methylation-associated biomarkers or regulatory features at the proteome scale.
Analysis Workflow
The Lysine Methylation Analysis Service at MtoZ Biolabs follows a systematic and optimized workflow:
1. Sample Preparation: Extraction of proteins under optimized conditions to retain modification integrity.
2. Enzymatic Digestion: Proteins are digested with trypsin or other proteases to generate peptides for downstream enrichment.
3. Enrichment of Modified Peptides: Antibody-based affinity enrichment or chemical strategies are applied to selectively capture lysine-methylated peptides.
4. LC-MS/MS Detection: High-resolution mass spectrometry platforms provide accurate mapping of methylation states and sites.
5. Bioinformatics Processing: Computational pipelines annotate methylation states, quantify site-specific changes, and interpret biological impact.
6. Quality Assessment: QC evaluation with replicate measurements ensures reliability of the final dataset.
Figure 2. Lysine Methylation Analysis Workflow
Sample Submission Suggestions
MtoZ Biolabs supports a wide range of sample types for lysine methylation analysis:
Serum/Plasma: ≥100 µL
Urine: ≥2 mL
Animal Tissue: ≥200 mg
Plant Tissue: ≥10 g
Microorganisms: ≥500 µL
Protein Extracts: ≥5 mg
Cells: ≥1 × 108
Samples should be frozen at -80°C and shipped on dry ice. Avoid detergents, glycerol, or salts that may interfere with downstream LC-MS/MS analysis. If you need further details, our technical support team is happy to assist and provide comprehensive guidance on sample submission.
Applications
Our Lysine Methylation Analysis Service provides insights across multiple research domains:
· Epigenetics: Profiling histone lysine methylation to study chromatin regulation and transcriptional control.
· Cancer Biology: Investigating aberrant methylation events linked to tumorigenesis and drug resistance.
· Neurobiology: Exploring lysine methylation in neuronal gene expression and neurological disorders.
· Immunology: Characterizing methylation-driven regulation of immune signaling pathways.
· Drug Discovery: Identifying novel therapeutic targets through systematic analysis of lysine methylation patterns.
Why Choose MtoZ Biolabs?
· Advanced PTM Analysis Platform: Integrated with state-of-the-art Orbitrap systems and optimized enrichment methods for reliable, high-resolution results.
· High Data Quality: Deep proteome coverage, strict QC measures, and bioinformatics interpretation that translate complex data into actionable insights.
· Broad Sample Compatibility: From plasma and tissues to microorganisms and cells, our workflows are validated across diverse biological materials.
· Customized Solutions: Flexible experimental design and tailored bioinformatics reports adapted to specific research goals and publication requirements.
· Transparent Pricing: One-time charge model with no hidden fees, ensuring cost clarity for research budgets.
FAQ
Q1: How can lysine methylation states such as mono, di, and tri methylation be distinguished by mass spectrometry?
High-resolution MS can differentiate methylation states based on precise mass shifts. At MtoZ Biolabs, Orbitrap instruments coupled with optimized fragmentation methods ensure accurate classification of methylation levels at specific lysine residues.
Q2: What challenges exist in analyzing non-histone lysine methylation?
Non-histone proteins often contain low-abundance or transient modifications that are harder to detect. Our enrichment and bioinformatics pipelines are optimized to capture these modifications reliably, extending analysis beyond histones to signaling and structural proteins.
Lysine methylation is a fundamental regulatory mechanism that influences gene expression, chromatin dynamics, and cellular signaling. Its complexity and diversity require advanced analytical workflows to resolve. MtoZ Biolabs provides a comprehensive Lysine Methylation Analysis Service that integrates enrichment strategies, high-resolution mass spectrometry, and bioinformatics expertise. By delivering reproducible and biologically meaningful results, we support researchers in academia, biotechnology, and pharmaceutical industries to uncover the regulatory functions of lysine methylation and accelerate scientific discovery.