Dimethylation Analysis Service
MtoZ Biolabs provides Dimethylation Analysis Service to deliver accurate identification and quantification of protein dimethylation events. Our workflows integrate optimized enrichment strategies with Orbitrap-based LC-MS/MS platforms, ensuring sensitive recovery of dimethylated peptides and reproducible datasets. For quantitative analysis, we offer TMT and iTRAQ labeling for multiplexed comparisons as well as label-free quantification for large-scale studies. These complementary approaches allow researchers to track biologically relevant changes in dimethylation across different experimental conditions.
What is Dimethylation?
Protein dimethylation is a frequent post-translational modification involving the addition of two methyl groups to lysine (Lys) or arginine (Arg) residues. This modification produces a +28 Da mass increase that can be precisely detected by high-resolution mass spectrometry.
Dimethylation occurs in two main forms: asymmetric dimethylation (ADMA) and symmetric dimethylation (SDMA). Each has distinct biological roles. ADMA is closely linked to RNA metabolism, signal transduction, and immune regulation, while SDMA plays a central role in chromatin remodeling and transcriptional control. Through these mechanisms, dimethylation serves as a crucial regulator of gene expression and cellular function. Aberrant dimethylation has been associated with cancer progression, autoimmune conditions, and neurological disorders, highlighting its biomedical significance.
Figure 1. Lysine Dimethylation
Services at MtoZ Biolabs
1. Targeted Protein Dimethylation Analysis
MtoZ Biolabs provides targeted dimethylation analysis for client-defined proteins, offering precise determination of dimethylated sites and quantitative assessment of modification levels on specific targets. This service is suitable for projects centered on individual regulatory proteins, targeted modification studies, or evaluation of dimethylation changes associated with specific treatments or biological models.
2. Dimethylation Proteomics
For broader investigations, MtoZ Biolabs offers dimethylation proteomics to characterize dimethylation patterns across the entire proteome. This service supports large-scale comparative studies, discovery-driven research, and the identification of dimethylation events relevant to signaling pathways, disease mechanisms, or biomarker exploration in complex biological samples.
Why Choose MtoZ Biolabs?
✔ Comprehensive PTM Platform: Our advanced analysis platform ensures reliable, accurate, and efficient dimethylation profiling.
✔ High Data Quality: Strict QC procedures and deep coverage guarantee reproducibility. Our bioinformatics solutions integrate PTM datasets to deliver comprehensive, publication-ready reports.
✔ Versatile Sample Support: Compatible with a wide range of biological matrices including cells, tissues, blood, and body fluids, accommodating diverse project requirements.
✔ Tailored Research Solutions: Flexible experimental designs and personalized data interpretation aligned with client-specific goals.
✔ Transparent Pricing: One-time charge policy with no hidden fees.
Sample Submission Suggestions
MtoZ Biolabs accepts a variety of biological samples for dimethylation analysis, including:
Serum/Plasma: ≥100 µL
Urine: ≥2 mL
Animal Tissue: ≥200 mg
Plant Tissue: ≥10 g
Microorganisms: ≥500 µL
Protein Extracts: ≥5 mg
Cells: ≥1 × 108
Samples should be stored at -80°C and shipped on dry ice. Avoid detergents, salts, or stabilizers that may interfere with LC-MS/MS. A detailed submission guide is available, and our scientific team can provide tailored consultation.
Applications
The Dimethylation Analysis Service provides insights across a wide range of studies:
• Epigenetics: Mapping histone dimethylation to investigate chromatin structure and transcriptional regulation.
• Oncology: Identifying dimethylation signatures that influence tumor progression and therapeutic resistance.
• RNA Biology: Exploring the role of asymmetric dimethylation in RNA splicing, processing, and stability.
• Immunology: Studying PRMT-mediated dimethylation in immune cell signaling and regulation.
• Drug Discovery: Supporting the development of small molecules that target PRMTs or dimethylation-dependent pathways.
What Could be Included in the Report?
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment
4. Data Analysis, Preprocessing, and Estimation
5. Bioinformatics Analysis
6. Raw Data Files
MtoZ Biolabs provides Dimethylation Analysis Service with an emphasis on accuracy, reproducibility, and flexibility. By combining selective enrichment, high-resolution mass spectrometry, and advanced bioinformatics, we deliver actionable datasets that support research in epigenetics, oncology, immunology, and beyond.
In addition to dimethylation, we also provide services for monomethylation, trimethylation, and a wide range of other PTMs. Contact our team to discuss how our expertise can accelerate your research.