Methylated Peptide Enrichment Service

MtoZ Biolabs offers Methylated Peptide Enrichment Service using immunoaffinity purification and hydrophilic interaction chromatography (HILIC) to selectively capture methylated peptides from complex mixtures. Since protein methylation often occurs at low abundance and low stoichiometry, enrichment prior to analysis is essential to achieve comprehensive coverage. Our service provides enriched peptide pools ready for downstream proteomics, enabling accurate mapping, quantification, and functional interpretation of protein methylation.

 

Protein methylation is a critical post-translational modification where lysine or arginine residues are modified by methyltransferases. Histone methylation has been extensively studied for its role in gene regulation and chromatin remodeling, while non-histone protein methylation is increasingly recognized in signaling, metabolism, and disease pathways. Methylated Peptide Enrichment Service helps researchers overcome the challenges of low-abundance methylation events, generating high-quality data for both histone and non-histone targets.

Sun, M. et al. Anal. Chem. 2025.

Figure 1. Workflow of Methylated Peptide Enrichment

 

Services at MtoZ Biolabs

MtoZ Biolabs applies enrichment strategies tailored to maximize specificity and sensitivity. Immunoaffinity purification is carried out using highly specific antibodies that recognize monomethyl arginine, asymmetric dimethyl arginine, and mono-, di-, and trimethyl lysine residues. These antibodies ensure selective capture of methylated peptides with high reproducibility. In addition, HILIC (hydrophilic interaction chromatography) is employed as a complementary method, offering a sequence-independent approach that captures diverse methylation types without restriction to peptide motifs.

 

Analysis Workflow

1. Sample Preparation: Extraction from cells, tissues, serum, plasma, exosomes, or microbial samples.

2. Protein Digestion: Trypsin cleavage generates peptides, exposing potential methylation residues.

3. Optional HILIC Fractionation: Reduces sample complexity and enhances detection of rare modifications.

4. Antibody-Based Enrichment: Immunoaffinity purification with validated antibodies against mono-, di-, and trimethyl Lys and Arg residues.

5. Downstream Options: Enriched peptides can be directed to LC-MS/MS, quantitative proteomics, or PTM cross-talk studies.

6. Reporting: Includes enrichment efficiency assessment, and if combined with analysis, detailed reports on methylation sites and pathway-level insights.

 

Why Choose MtoZ Biolabs?

✔ High Specificity: Immunoaffinity purification with validated antibodies targeting mono-, di-, and trimethyl residues.

✔ Broad Coverage: Integration of HILIC ensures detection beyond sequence or motif constraints.

✔ Flexible Integration: Enriched peptides are compatible with quantitative workflows and cross-PTM studies.

✔ Comprehensive Support: From sample submission to enrichment QC and optional downstream analysis.

✔ One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

 

Sample Submission Suggestions

 

Note: Provide details on sample collection and handling. If you need further details, our technical support team is happy to assist and provide comprehensive guidance on sample submission.

 

FAQ

Q1: Why combine antibody enrichment with HILIC?

While antibodies ensure high specificity, HILIC expands coverage by enriching methylated peptides without sequence restrictions, improving overall detection.

 

Q2: Can different methylation types be distinguished after enrichment?

Yes. Enrichment captures the peptides, and downstream mass spectrometry differentiates between mono-, di-, and trimethylation states with site-specific resolution.

 

Methylated Peptide Enrichment Service at MtoZ Biolabs empowers researchers with reliable access to enriched peptide pools that open new possibilities for functional studies of protein methylation. Our expertise ensures smooth integration with downstream proteomic workflows and bioinformatics pipelines, providing data that supports discoveries in biology, medicine, and biotechnology. Beyond methylation, MtoZ Biolabs offers a full portfolio of PTM-omics services, including phosphorylation, acetylation, ubiquitination, and succinylation. Contact us today to discuss how our Methylated Peptide Enrichment Service can accelerate your research goals.