Platforms

MtoZ Biolabs is a professional CRO company focusing on proteomics, mass spectrometry analysis, and multi-omics detection. With advanced mass spectrometry platforms and a complete experimental system, we provide systematic and standardized detection and analysis solutions for global scientific and industrial clients. The company is committed to supporting scientific researchers with high-quality data to explore the molecular mechanisms of protein post-translational modifications (PTMs), thereby promoting drug development, disease research, and fundamental life science studies. The technology platform of MtoZ Biolabs not only includes common mass spectrometry methods but also integrates multiple modified peptide enrichment strategies, bioinformatics algorithms, and quantitative analysis approaches, offering clients one-stop detection and analytical services.

 

Services at MtoZ Biolabs

MtoZ Biolabs’ technology platform focuses on post-translational modification research, building a complete chain from modified peptide enrichment, quantitative analysis, and mass spectrometry detection to bioinformatics interpretation. Each step complements the others, ensuring the reliability and scientific value of the results. Our technology platform covers the following aspects:

 

Platform	Method	Description
Modified Peptide Enrichment  Service	SAX & SCX-Based Modified Peptide Enrichment Service	Separation of modified peptides based on charge differences, suitable for initial fractionation of phosphorylated, acetylated, and other charged modifications.
	HILIC-Based Modified Peptide Enrichment Service	Separation based on peptide polarity, especially suitable for glycosylation and other hydrophilic modifications.
	RPLC-Based Modified Peptide Enrichment Service	High-resolution separation commonly used before mass spectrometry, often combined with other enrichment methods.
	IMAC-Based Modified Peptide Enrichment Service	Selective enrichment of phosphorylated peptides using affinity between metal ions and phosphate groups.
	MOAC-Based Modified Peptide Enrichment Service	Uses TiO₂, ZrO₂, etc., suitable for capturing low-abundance phosphorylated peptides.
	Immunoaffinity Precipitation-Based Modified Peptide Enrichment Service	Specific enrichment of acetylation, methylation, ubiquitination, and other modifications using antibodies.
	M-LAC-Based Modified Peptide Enrichment Service	Broad glycan enrichment using multiple lectins.
Quantitative  Post-Translational Modification Service	SILAC-Based Quantitative PTM Service	Accurate cell-level comparison through metabolic labeling.
	TRAQ/TMT-Based Quantitative PTM Service	Multiplexed quantification of multiple samples in one experiment.
	Label-Free-Based Quantitative PTM Service	No labeling required, suitable for large-scale exploratory studies.
	DIA/SWATH-Based Quantitative PTM Service	High coverage and low missing values, suitable for clinical samples.
MS-Based  Post-Translational Modification Analysis Service	Top-Down MS-Based Post-Translational Modification Analysis Service	Direct analysis of intact proteins without digestion, retaining sequence and overall PTM distribution, suitable for isoform and multi-modification studies.
	Bottom-Up MS-Based Post-Translational Modification Analysis Service	Proteins digested into peptides, followed by LC-MS/MS, the most common approach for large-scale identification and quantification of sites.
	Middle-Down MS-Based Post-Translational Modification Analysis Service	Partial digestion into medium-length fragments, balancing global structure and site-level details, suitable for regional modification analysis.
Protein Post-Translational Modification Bioinformatics Analysis Service	Post-Translational Modification Crosstalk Prediction Service	Studies interactions among modifications.
	Post-Translational Modification Site Prediction Service	Predicts potential modification sites via experimental and computational approaches.
	Post-Translational Modification Site Motif Analysis Service	Identifies common sequence motifs of modifications.
	Post-Translational Modification Functional Analysis Service	Maps modifications to cellular functions and disease mechanisms.
Protein Microarray-Based PTM Analysis Service		enables parallel investigation of protein expression, binding events, and post-translational modifications.

Analysis Workflow

1. Sample Preparation: Clients provide cells, tissues, or body fluids that meet requirements, followed by extraction and pretreatment.

2. Modified Peptide Enrichment: Target modifications selectively enriched according to research goals.

3. Mass Spectrometry Detection: High-resolution LC-MS/MS used for data acquisition.

4. Data Processing: Raw data undergoes quality control, peptide identification, and modification localization.

5. Bioinformatics Analysis: Quantitative comparison, functional enrichment, and pathway analysis with visualization.

 

Figure 1. Workflow of Bottom-Up PTM Proteomics

 

Service Advantages

1. Advanced Platform

MtoZ Biolabs is equipped with multiple high-resolution mass spectrometry systems, including Thermo Fisher Orbitrap Fusion Lumos, Q Exactive HF, and TripleTOF platforms, integrated with Nano-LC systems. This setup supports diverse research needs, from low-abundance modification detection to large-scale quantitative comparisons.

 

2. Experienced Team

MtoZ Biolabs has a technical team with long-term experience in proteomics and post-translational modification research, familiar with the handling and detection characteristics of different sample types. With years of accumulated expertise, we can design appropriate experimental routes based on client requirements, ensuring reliable and interpretable results.

 

3. One-Time Charge

Our pricing is transparent, with no hidden fees or additional costs.

 

Applications

✔️ Basic Research: Explore the molecular mechanisms of protein modifications and their roles in signal transduction, metabolic regulation, and gene expression.

✔️ Disease Research: Investigate abnormal PTMs in cancer, neurodegenerative diseases, autoimmune disorders, etc., providing evidence for biomarker screening.

✔️ Drug Development: Monitor dynamic changes in modifications to identify potential targets and mechanisms, accelerating drug discovery and validation.

✔️ Biopharmaceutical Quality Control: Applied in antibody, recombinant protein, and other product development for structural characterization and functional verification, ensuring product safety and efficacy.

 

With a complete technology platform and a professional team, MtoZ Biolabs provides systematic PTM research solutions for scientific and industrial clients. From modified peptide enrichment to mass spectrometry analysis, and from data interpretation to application translation, we are committed to offering reliable support for your research. Contact us to advance proteomics and life science exploration together.