Acetylomics Data Analysis Service
Acetylomics Data Analysis Service provides systematic bioinformatics support for the identification, quantification, and interpretation of protein acetylation events. Protein acetylation is one of the most widespread post-translational modifications and occurs when acetyl groups are added to lysine residues by acetyltransferases. This modification is highly dynamic and reversible, shaping gene expression, chromatin remodeling, enzymatic activity, and signal transduction. Because of its central role in regulating cellular physiology, acetylation has been directly linked to cancer progression, metabolic disorders, aging, and neurodegenerative diseases.
At MtoZ Biolabs, we transform raw LC–MS/MS datasets into biologically meaningful results using advanced computational workflows and professional interpretation. Our Acetylomics Data Analysis Service is compatible with different data types, including files generated from antibody-based acetylation enrichment, and supports multiple quantitative strategies such as label-free, TMT, and iTRAQ. Beyond the detection of acetylation events, our service explores their regulatory significance, helping clients understand how acetylation modifies protein function and contributes to health and disease.
Services at MtoZ Biolabs
MtoZ Biolabs offers a wide spectrum of analytical capabilities to address different levels of acetylomics research:
· Data Processing and Quality Evaluation
Conversion of raw MS files into analysis-ready formats, removal of background noise, and evaluation of peptide length, protein coverage, and peptide uniqueness. These steps ensure that the dataset is of sufficient quality for downstream interpretation.
· Acetylation Site Identification
Detection and localization of lysine acetylation events using curated protein databases, coupled with statistical confidence scoring. This step provides high-resolution mapping of both histone and non-histone protein modifications.
· Quantitative Analysis of Acetylated Proteins
Measurement of protein and peptide abundances across different conditions using label-free, TMT, or iTRAQ methods. Statistical tests and principal component analysis are applied to identify significantly changing acetylation events and to reveal biological trends.
· Motif Discovery and Sequence Analysis
Identification of conserved amino acid patterns surrounding acetylation sites, highlighting potential recognition sequences for acetyltransferases and deacetylases.
· Functional Annotation and Pathway Enrichment
Comprehensive annotation of acetylated proteins using Gene Ontology, KEGG pathways, and orthology-based classification. These analyses connect acetylation events to cellular processes such as metabolism, transcriptional control, and DNA repair.
· Protein Network and Domain Analysis
Construction of protein interaction networks and prediction of functional domains to illustrate how acetylated proteins contribute to regulatory complexes and structural features.
· Integrated Bioinformatics Analysis
Correlation of acetylation data with other post-translational modifications, such as phosphorylation and ubiquitination, for a broader view of proteome regulation. This integrative approach enables a systems-level understanding of cellular control mechanisms.
If existing analyses fail to meet your expectations, MtoZ Biolabs provides fully integrated acetylomics solutions, ranging from sample preparation and mass spectrometry acquisition to bioinformatics interpretation, ensuring accurate site identification and reliable data output.
Analysis Workflow
Why Choose MtoZ Biolabs?
✔ Advanced Platforms: We process Orbitrap and Q-TOF mass spectrometry datasets with high precision, ensuring reproducible and reliable acetylomics analysis results.
✔ Comprehensive Coverage: Our workflows cover both histone and non-histone acetylation, providing a full view of acetylation-driven biological processes.
✔ Flexible Quantification: We support multiple quantitative strategies, including label-free, TMT, and iTRAQ, adaptable to different study designs and project objectives.
✔ Biological Insight: Beyond site detection, we offer motif discovery, functional annotation, and pathway mapping, ensuring biologically meaningful interpretation of acetylomics data.
✔ One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
Applications
MtoZ Biolabs’ Acetylomics Data Analysis Service goes beyond site identification by delivering comprehensive bioinformatics insights that help researchers uncover the biological significance of acetylation. Through advanced computational analysis, our service supports the following key applications:
1. Differential Site Identification and Comparison
Accurately detect and quantify differential acetylation sites between experimental groups, providing robust data support for functional studies and downstream validation.
2. Functional Annotation and Pathway Enrichment
Integrate Gene Ontology (GO) and KEGG pathway analysis to reveal molecular functions, biological processes, and signaling pathways associated with acetylated proteins.
3. Subcellular Localization and Network Modeling
Map differentially acetylated proteins to subcellular structures and incorporate protein–protein interaction data to build regulatory networks for system-level insights.
4. Protein Domain and Epigenetic Regulation Analysis
Characterize enriched protein domains and histone acetylation patterns, advancing the understanding of acetylation in chromatin remodeling and transcriptional regulation.
Gao, J. et al. Front. Med. 2023.
Figure 1. Multi-Dimensional Acetylomics Data Interpretation
What Could be Included in the Report?
· A full analysis report including methodology, results, and biological interpretation
· Comprehensive tables of acetylated proteins and modification sites with confidence scores
· Quantitative comparisons of acetylation levels across experimental conditions
· Visualization outputs including heatmaps, volcano plots, principal component analysis, protein interaction networks, and pathway enrichment diagrams
· Motif analysis results identifying conserved sequence features of acetylation sites
· Raw and processed datasets provided in standard formats, including RAW, mzML, FASTA, and Excel files
MtoZ Biolabs’ Acetylomics Data Analysis Service provides a structured, systematic approach to extracting meaningful insights from complex proteomics datasets. By combining robust bioinformatics pipelines with biological interpretation, we empower researchers to understand the role of acetylation in cellular regulation and disease.
Contact us today to learn how our acetylomics expertise can support your research and complement our full portfolio of post-translational proteomics data analysis services.