Top-Down MS-Based Post-Translational Modification Analysis Service
MtoZ Biolabs provides Top-Down MS-Based Post-Translational Modification (PTM) Analysis Service to achieve direct, high-resolution characterization of intact proteins and their modification states. This advanced strategy allows researchers to study full-length proteins without enzymatic digestion, preserving the natural connectivity between PTMs and the protein backbone.
Leveraging our Orbitrap and Fourier Transform Ion Cyclotron Resonance (FT-ICR) mass spectrometry platforms, MtoZ Biolabs offers unparalleled mass accuracy, sequence coverage, and PTM localization capability. Our top-down workflows enable the mapping of proteoforms, quantification of multiple modified states, and identification of rare or combinatorial modifications that are often lost in traditional peptide-based proteomics.
Overview
Top-down mass spectrometry is a proteomics method that analyzes intact protein ions to identify sequences, detect modifications, and determine proteoform heterogeneity. This strategy allows direct visualization of the full protein backbone, enabling simultaneous detection of all modification states.
Fragmentation by ECD or ETD preserves fragile modifications such as phosphorylation, acetylation, and glycosylation, resulting in confident site localization and high sequence coverage. Top-down MS is particularly suitable for analyzing small to medium-sized proteins, histones, and regulatory enzymes where PTMs co-occur and dynamically influence function.
Through top-down proteomics, MtoZ Biolabs provides researchers with comprehensive insight into how complex PTM combinations define protein behavior, offering both structural and functional understanding within a single experiment.
Analysis Workflow
The Top-Down MS-Based Post-Translational Modification Analysis Service at MtoZ Biolabs follows a validated analytical pipeline for maximum precision and reproducibility.
1. Sample Preparation: High-purity protein extraction under optimized conditions to preserve native modifications.
2. Protein Separation: Intact proteins are separated by liquid chromatography or capillary electrophoresis to reduce sample complexity.
3. Mass Spectrometry Detection: Orbitrap or FT-ICR instruments acquire high-resolution mass spectra for intact proteins.
4. Fragmentation and PTM Mapping: ECD and ETD dissociation identify modification sites while retaining labile PTMs.
5. Proteoform Identification and Quantification: Advanced algorithms deconvolute spectra and quantify proteoform abundance.
6. Bioinformatics and Data Validation: Comprehensive software pipelines validate assignments and integrate results into biological context.
Sun, M. et al. Cell Biosci. 2022.
Figure 1. Schematic Workflow of Top-Down Proteomics
Why Choose MtoZ Biolabs
✅ Advanced Analysis Platform: Orbitrap and FT-ICR systems deliver unmatched mass accuracy and resolution.
✅ Extensive PTM Expertise: Proven track record in multi-PTM proteomics and structural biology.
✅ Strict Data Quality Control: Each dataset is verified through multi-step validation to ensure reproducibility.
✅ Versatile Sample Compatibility: Applicable to purified proteins, tissues, lysates, and biological fluids.
✅ Customizable Analytical Solutions: Tailored workflows and interpretation based on individual project goals.
✅ One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
Applications
The Top-Down MS-Based Post-Translational Modification Analysis Service supports a broad range of research and industrial applications:
● Epigenetics: Characterizing histone proteoforms and combinatorial PTMs.
● Signal Transduction: Mapping regulatory modifications in kinases and transcription factors.
● Drug Mechanism Studies: Profiling PTM-dependent proteoform alterations in response to drug treatment.
● Biotherapeutics Characterization: Assessing integrity and modification heterogeneity of recombinant proteins.
● Proteoform Discovery: Revealing structural variants and novel PTMs in complex biological systems.
Top-Down MS-Based Post-Translational Modification Analysis Service at MtoZ Biolabs provides accurate, high-resolution characterization of intact proteins and their post-translational modifications. By combining advanced instrumentation, optimized analytical workflows, and expert bioinformatics interpretation, we help researchers unravel proteoform complexity and gain deeper molecular insights.
Our scientific team is dedicated to supporting diverse PTM research with reliable data and customized analytical solutions. To discuss your project, please contact MtoZ Biolabs for professional consultation.
What Could be Included in the Report?
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment
4. Data Analysis, Preprocessing, and Estimation
5. Bioinformatics Analysis
6. Raw Data Files