Monomethylation Analysis Service
MtoZ Biolabs provides Monomethylation Analysis Service to support accurate detection and quantification of monomethylation events. By combining optimized immunoaffinity enrichment with Orbitrap-based LC-MS/MS, we achieve sensitive recovery of low-abundance peptides. For quantitative analysis, we employ TMT and iTRAQ labeling to enable reliable cross-sample comparison and multiplexed analysis, while label-free quantification offers flexibility for large-scale studies. These complementary strategies ensure reproducible datasets that reveal biologically meaningful changes in monomethylation across diverse conditions.
What is Monomethylation?
Monomethylation is a post-translational modification (PTM) in which a single methyl group is added to a lysine or arginine residue. This modification introduces a precise mass increase of +14 Da relative to the unmodified amino acid, which can be accurately detected by high-resolution mass spectrometry. Monomethylation is often associated with enhancer priming in histones, as well as regulation of protein-protein interactions and signaling dynamics in non-histone proteins. Its dysregulation has been linked to cancer, immune disorders, and neurological diseases, underscoring its importance in biomedical research.
Figure 1. Lysine Monomethylation
Services at MtoZ Biolabs
1. Targeted Protein Monomethylation Analysis
MtoZ Biolabs offers targeted monomethylation analysis for client-specified proteins, delivering accurate site-level identification and quantitative evaluation of monomethylation on defined targets. This service is appropriate for studies requiring focused assessment of specific proteins, such as target verification, pathway-related investigations, or monitoring modification changes under controlled experimental settings.
2. Monomethylation Proteomics
For system-wide characterization, MtoZ Biolabs provides monomethylation proteomics to profile monomethylation events across all proteins in complex biological samples. This global approach supports discovery-oriented projects, comparative analyses across conditions or disease states, and the identification of monomethylation signatures with potential functional or biomarker relevance.
Sample Submission Suggestions
MtoZ Biolabs accepts a wide range of biological samples for monomethylation analysis, including:
Serum/Plasma: ≥100 µL
Urine: ≥2 mL
Animal Tissue: ≥200 mg
Plant Tissue: ≥10 g
Microorganisms: ≥500 µL
Protein Extracts: ≥5 mg
Cells: ≥1 × 108
Samples should be stored at -80°C and shipped on dry ice. Avoid detergents, salts, or stabilizers that may interfere with LC-MS/MS analysis.
Applications
Monomethylation Analysis Service supports a wide range of research and translational studies. The following represent typical areas of application:
1. Epigenetics and Gene Regulation
Monomethylation plays a pivotal role in chromatin remodeling and transcriptional regulation. Accurate site-specific detection helps researchers uncover the molecular mechanisms underlying gene expression control.
2. Stem Cell Differentiation and Development
During stem cell differentiation and tissue development, monomethylation marks undergo dynamic changes. Comprehensive analysis provides insights into key regulatory signals that determine cell fate.
3. Disease Mechanisms and Biomarker Discovery
Aberrant monomethylation is closely associated with cancer, neurodegenerative disorders, and immune-related diseases. In-depth profiling enables the exploration of disease mechanisms and the identification of potential biomarkers.
4. Drug Discovery and Target Validation
Monomethylation can serve as both a novel drug target and an indicator for therapeutic efficacy. By applying advanced quantitative strategies, researchers can obtain reliable data to accelerate drug development and mechanism validation.
Why Choose MtoZ Biolabs?
✅ Advanced Mass Spectrometry Platforms: Orbitrap Fusion Lumos and Q Exactive HF ensure sensitive and precise detection.
✅ Transparent Pricing: One-time charge model with no hidden fees.
✅ High Data Quality: Rigorous QC and deep coverage provide reproducible results.
✅ Broad Sample Compatibility: Validated workflows for cells, tissues, body fluids, and purified protein extracts.
✅ Customized Solutions: Flexible study design and bioinformatics support tailored to project goals.
What Could be Included in the Report?
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment
4. Data Analysis, Preprocessing, and Estimation
5. Bioinformatics Analysis
6. Raw Data Files
Monomethylation has emerged as a crucial layer of protein regulation, linking chromatin remodeling, transcriptional control, and cellular signaling. With our Monomethylation Analysis Service, researchers gain the precision and reliability needed to study this modification in depth. Beyond monomethylation, MtoZ Biolabs provides a full suite of PTM analysis solutions, empowering discoveries that span from molecular mechanisms to therapeutic innovation. We invite you to connect with our team to design a workflow that fits your research goals.