Phosphoproteomics Service

Phosphoproteomics focuses on the large-scale study of protein phosphorylation, a dynamic and reversible modification that regulates cellular signaling, disease progression, and drug response. By mapping phosphorylation sites and quantifying their changes, phosphoproteomics reveals key regulatory mechanisms underlying biological processes.

 

MtoZ Biolabs offers systematic Phosphoproteomics Service powered by Thermo Fisher Q Exactive HF, Orbitrap Fusion, and Orbitrap Fusion Lumos mass spectrometers combined with Nano-LC. With high sensitivity and resolution, our platform enables precise site identification, quantitative comparisons, and pathway-level insights, ensuring reliable and reproducible results. Through integrated workflows and advanced bioinformatics, we deliver comprehensive phosphorylation profiling to support biomedical research, drug discovery, and agricultural studies.

 

Services at MtoZ Biolabs

MtoZ Biolabs provides a full suite of phosphoproteomics services tailored to diverse research objectives:

● Phosphopeptide Enrichment Service

Selective capture of phosphorylated peptides using TiO₂, IMAC, and anti-phosphotyrosine antibodies to improve low-abundance detection.

● Phosphorylation Site Identification Service

Precise localization of phosphorylation on Ser, Thr, and Tyr residues with high-confidence coverage.

● Tyrosine Phosphoproteomics Service

Focused analysis of tyrosine phosphorylation events critical for cancer, immune pathways, and therapeutic targeting.

● Quantitative Phosphoproteomics Service

Label-Free, TMT/iTRAQ, and DIA quantification methods provide flexible and accurate phosphorylation dynamics.

● Phosphoproteomics Data Analysis Service

Comprehensive bioinformatics for differential expression, kinase activity prediction, pathway enrichment, and statistical validation.

● Single Cell Phosphoproteomics Service

High-sensitivity workflows enable phosphorylation profiling at the single-cell level, uncovering signaling heterogeneity.

● Multi-Channel Phosphorylated Protein Analysis Service

Parallel profiling of multiple phosphorylation channels to reveal complex signaling networks.

 

Analysis Workflow

1. Protein Extraction: Isolation from cells, tissues, serum, plasma, or exosomes with strict quality control.

2. Enzymatic Digestion: High-purity trypsin digestion to generate peptides for LC-MS/MS.

3. Phosphopeptide Enrichment: TiO₂, IMAC, or antibody-based enrichment for enhanced detection.

4. Mass Spectrometry Detection: High-resolution Orbitrap MS combined with Nano-LC.

5. Data Analysis: Site identification, quantification, pathway annotation, kinase activity prediction.

6. Report Delivery: Comprehensive results including site distribution, quantitative changes, functional annotations, and visualized outputs.

 

Figure 1. Workflow of Phosphoproteomics Analysis

 

Why Choose MtoZ Biolabs?

✔ Advanced Platform: Orbitrap MS with Nano-LC delivers high sensitivity and resolution.
✔ Diverse Enrichment: TiO₂, IMAC, and antibody-based methods improve depth and coverage.
✔ Flexible Quantification: Label-Free, TMT/iTRAQ, and DIA approaches meet diverse needs.
✔ Expert Bioinformatics: Mainstream software ensures accurate analysis and interpretation.
✔ Integrated Workflow: From sample preparation to reporting, ensuring reproducibility.
✔ Transparent Pricing: One-time charge policy with no hidden fees.

 

Sample Submission Suggestions

Sample Type		Recommended Quantity	Minimum Quantity
Animal Tissue	Soft tissues (e.g., brain, heart, liver, spleen, lung, kidney, muscle, skin)	100 mg	50 mg
	Hard tissues (e.g., bone, cartilage, hair)	5 g	3 g
	Tumor tissue (tumor or adjacent connective tissue-containing samples)	100 mg	50 mg
Plant Tissue	Soft plant tissue (e.g., leaves and flowers of woody plants, grasses, algae, ferns, large fungi)	2 g	1 g
	Hard plant tissue (e.g., roots, bark, branches, seeds, fruits)	5 g	2 g
Cell Pellet	Adherent or suspension cells	2x107	1x107
Microbes	Common bacteria, fungal cells (cell pellets)	500 mg or 500 μL	250 mg or 250 μL
Body Fluids	Serum/plasma (without high-abundance protein depletion)	50 μL	20 μL
Protein Solution	User-extracted protein solution (composition must be provided in advance)	2 mg	1 mg
Other Special Samples		Please Inquire Separately

 

Note: Provide details on sample collection and handling. If you need further details, our technical support team is happy to assist and provide comprehensive guidance on sample submission.

 

Applications

Our phosphoproteomics services support a broad range of applications across life sciences and industry:

1. Disease Mechanism Research: Mapping signaling pathways in cancer, cardiovascular, and neurological diseases.

2. Biomarker Discovery: Identifying phosphorylation-based biomarkers for diagnosis, prognosis, and therapeutic monitoring.

3. Drug Development: Evaluating kinase activity, drug-target interactions, and treatment responses.

4. Precision Medicine: Profiling patient-specific phosphorylation dynamics for personalized strategies.

5. Plant and Agricultural Research: Investigating stress responses and regulatory mechanisms in crops.

 

Phosphoproteomics Service at MtoZ Biolabs delivers reliable site identification, quantitative insights, and pathway analysis powered by advanced Orbitrap platforms. Contact us today to advance your research with our Phosphoproteomics Service.

 

What Could be Included in the Report?

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Total Ion Chromatogram & Quality Control Assessment

4. Data Analysis, Preprocessing, and Estimation

5. Bioinformatics Analysis

6. Raw Data Files