Redox Proteomics Analysis Service
Redox proteomics is a scientific field that systematically investigates the dynamic changes and regulatory mechanisms of proteins between oxidized and reduced states. This research analyzes the structural and functional variations of proteins under different redox conditions to reveal cellular responses involved in oxidative stress, metabolic regulation, and signal transduction. With the advancement of high-resolution mass spectrometry, redox proteomics has become an important approach for understanding cellular homeostasis, stress adaptation, and functional regulation, and is widely applied in life sciences, pharmaceutical research, environmental toxicology, and bioengineering.
Lennicke, C. et al. Proteimics, 2016.
Figure 1. Selected Redox Modifications of Proteins by ROS/RNS and Their Consequences.
Services at MtoZ Biolabs
Based on a high-resolution mass spectrometry (LC-MS/MS) platform and multidimensional chemical labeling strategies, MtoZ Biolabs has launched the redox proteomics analysis service which enables systematic profiling of various types, sites, and dynamic changes of redox-related protein modifications. This service integrates differential labeling, affinity enrichment, and quantitative mass spectrometry detection technologies to simultaneously identify and quantify multiple forms of redox modifications. By combining database searching with bioinformatics analysis, it provides researchers with reliable data support. MtoZ Biolabs offers services including, but not limited to, the following:
1. S-Nitrosylation
S-nitrosylation is a reversible redox modification in which a cysteine residue forms an S-NO bond with nitric oxide, regulating protein activity and function.
2. S-Glutathionylation
S-glutathionylation is a modification in which a protein thiol forms a mixed disulfide bond with glutathione, serving to maintain cellular redox balance and protect against oxidative damage.
Analysis Workflow
1. Sample Preparation and Protein Extraction
Total proteins are extracted from samples under antioxidant conditions to preserve the original redox modification state.
2. Chemical Labeling and Modification Protection
Differential labeling or specific chemical reactions are applied to stabilize redox modifications.
3. Enrichment and Purification
Affinity capture or specific chemical enrichment strategies are used to selectively isolate modified peptides.
4. Mass Spectrometry Detection and Data Acquisition
High-resolution LC-MS/MS is employed to detect peptides and acquire fragmentation spectra of redox modifications.
5. Data Analysis and Functional Annotation
Database searching is performed to identify modification sites and types, followed by functional annotation and pathway analysis.
Day, N J. et al. Antioxidants (Basel). 2021.
Figure 2. Workflow of Different Quantitative Redox Proteomics Approaches.
Sample Submission Suggestions
1. Sample Types
Acceptable samples include cells, tissues, serum, plasma, and microbial samples, as well as protein extracts or lyophilized powders.
Note: Strong oxidants or reducing agents should be avoided during sample processing to preserve the original redox modification state.
2. Sample Storage
Samples should be sealed and protected from light at -80°C for long-term storage or -20°C for short-term storage. Avoid repeated freeze-thaw cycles and air exposure to prevent protein oxidation or loss of modifications.
3. Sample Transportation
Liquid samples should be transported via cold chain. Lyophilized samples can be shipped at room temperature for short periods but should be protected from heat, humidity, and light to ensure sample stability and analytical accuracy.
Service Advantages
1. High-Resolution Detection Platform
Powered by an advanced LC-MS/MS system, enabling highly sensitive detection and precise quantification of multiple redox modification types.
2. Multi-Level Modification Identification
Capable of simultaneously analyzing S-nitrosylation, S-glutathionylation, disulfide bonds, and other redox modifications to reveal multidimensional regulatory features.
3. Rigorous Quality Control
A standardized quality control system is implemented throughout the workflow to ensure data accuracy and reproducibility.
4. Customized Analysis Solutions
Experimental strategies can be flexibly adjusted based on sample type and research objectives to meet diverse scientific requirements.
Applications
1. Cell Homeostasis Research
Redox proteomics analysis service can be used to evaluate the role of redox modifications in maintaining cellular homeostasis and regulatory balance.
2. Metabolic Engineering Optimization
By analyzing redox modification patterns in microorganisms, this service supports the optimization of metabolic pathways and enhancement of product yield.
3. Processing and Storage Studies
Redox proteomics analysis service can be applied to monitor oxidative modification changes during sample processing or storage.
4. Protein Complex Studies
By identifying redox-sensitive sites, the service helps assess how oxidative modifications affect the stability of protein complexes.
FAQ
Q1: Can This Service Perform Quantitative Analysis?
A1: Yes. Using TMT, iTRAQ, or label-free strategies, it enables comparative analysis of redox modification levels under different conditions.
Q2: Can Mass Spectrometry Distinguish between Different Types of Redox Modifications?
A2: Yes. By combining high-resolution LC-MS/MS with specific chemical labeling techniques, different modification types can be differentiated and modification sites accurately localized.
Q3: Can Redox Proteomics Be Integrated with Other Omics Approaches?
A3: Yes. It can be integrated with proteomics, phosphoproteomics, or metabolomics to uncover multi-layered regulatory networks.
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