Bacteria Glycosylate Analysis Service

Bacteria glycosylate analysis service aims to systematically characterize the glycan information carried by bacterial proteins during post-translational modification, including glycan types, distribution patterns, and modification sites. This analysis can reveal the roles of bacterial glycosylation in structural stability, host interactions, pathogenesis, and immune evasion, providing key data for pathogen research, vaccine development, resistance mechanism exploration, and synthetic biology applications. Since bacterial glycosylation is directly linked to protein function and biological effects, accurate detection is critical for both scientific research and industrial applications. Relying on advanced mass spectrometry and chromatography platforms, MtoZ Biolabs provides a high-quality bacteria glycosylate analysis service that delivers reliable data support to facilitate scientific breakthroughs and product development.

Poole, J. et al. Nat Rev Microbiol. 2018.

Figure 1. Bacterial Glycosylation Drives Pathogenesis and Immune Evasion

 

Services at MtoZ Biolabs

MtoZ Biolabs’ bacteria glycosylate analysis service is based on multi-dimensional technologies, covering different research needs from global glycan features to site-specific modification identification. The main technologies include:

1. Liquid Chromatography–Tandem Mass Spectrometry (LC-MS/MS)

LC-MS/MS is one of the core methods for bacterial glycosylation analysis. By separating glycopeptides or glycans in complex samples with high-performance liquid chromatography and detecting them with high-resolution mass spectrometry, glycosylation sites can be precisely identified and glycan structures confirmed. This method reveals modification types and distribution while supporting relative quantification, suitable for comparative studies under different conditions.

 

2. Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS)

MALDI-TOF MS is suitable for rapidly establishing glycan fingerprint profiles. It detects released glycans to provide global glycosylation information. This method is simple and efficient, especially for comparative screening of multiple sample batches.

 

3. High-Performance Liquid Chromatography (HPLC/UPLC-FLR)  

HPLC or UPLC-FLR combined with fluorescence labeling enables glycan separation and quantification. Fluorescent derivatization enhances detection sensitivity, providing clear data on glycan composition and abundance distribution. This method is commonly used for quality control and batch-to-batch consistency evaluation.

 

4. Capillary Electrophoresis (CE)

CE offers extremely high resolution in glycan isomer separation, complementing chromatography and mass spectrometry. It is especially valuable for detailed studies of complex glycans.

 

5. Enrichment and Derivatization Techniques

• Lectin affinity enrichment: selectively captures specific glycan types to enhance detection sensitivity.
• Chemical derivatization (e.g., permethylation, reductive amination): strengthens glycan signals and improves structural interpretation in mass spectrometry.

 

Analysis Workflow

1. Sample receipt and preparation: confirm sample information and research objectives, followed by basic pretreatment.

2. Sample pretreatment: glycan release, glycopeptide enrichment, or protein purification.

3. Separation and detection: HPLC, UPLC, or CE separation combined with mass spectrometry analysis.

4. Data analysis: identify glycan structures and glycosylation sites using databases and specialized software.

5. Result delivery: generate a complete report including experimental results, spectra, and integrated analysis.

 

Service Advantages

1. Multi-technology integration

We combine LC-MS/MS, MALDI-TOF, HPLC/UPLC-FLR, and CE to analyze bacterial glycosylation from multiple perspectives, ensuring completeness and diversity of results.

 

2. High resolution and sensitivity

With advanced mass spectrometry platforms and precise separation methods, our bacteria glycosylate analysis service can capture low-abundance modifications in complex samples, ensuring accuracy and reliability.

 

3. Flexible pretreatment strategies

Depending on sample type and research needs, different enrichment and derivatization methods can be applied to improve detection sensitivity and structural depth, avoiding loss of key information.

 

4. Customized research design

Our analysis pathways are tailored to client objectives, avoiding redundant steps, improving efficiency, and ensuring the service fits practical needs.

 

5. Transparent deliverables

We provide raw data, methodological descriptions, and detailed reports, ensuring clients have full access to results and can perform further validation or downstream analysis.

 

Applications

✔️ Pathogenesis research: reveal the role of glycosylation in bacterial adhesion, invasion, and immune evasion.

✔️ Resistance mechanism exploration: compare glycosylation patterns across strains to identify clues linked to resistance.

✔️ Vaccine development: analyze glycosylation features of bacterial surface antigens to support vaccine design.

✔️ Host–microbe interaction studies: investigate how glycosylation influences immune recognition and ecological adaptation.

✔️ Synthetic biology and metabolic engineering: optimize bacterial expression systems to improve product stability and functionality.

 

FAQs

Q1: How long does the bacteria glycosylate analysis service usually take?

A1: Typically 3–4 weeks, depending on sample complexity and project design.

 

Sample Submission Suggestions

1. Sample types: bacterial lysates, purified proteins, or secreted products.

2. Storage: liquid samples should be frozen and shipped on dry ice; lyophilized samples can be shipped at room temperature.

3. Recommended amount: at least 50–100 µg of total protein.

4. Notes: avoid high salt or strong detergents; please contact us if pretreatment guidance is needed.

 

Deliverables

1. Experimental workflow and methodology description

2. Glycan profiles and quantitative distribution data

3. Glycopeptide/glycosylation site identification results

4. Complete raw mass spectrometry data files

5. Comprehensive analysis report

 

Bacterial glycosylation analysis is a powerful tool for understanding bacterial function, elucidating pathogenic mechanisms, and advancing the development of novel therapies. With advanced analytical platforms and an experienced team, MtoZ Biolabs provides a systematic bacteria glycosylate analysis service that supports comprehensive research goals, and if you are seeking a reliable partner, we welcome you to contact us and accelerate your scientific and industrial projects toward success.