Acetylated Peptide Enrichment Service

MtoZ Biolabs offers Acetylated Peptide Enrichment Service powered by antibody-based affinity capture strategies. Our service enables researchers to overcome the challenge of low-abundance acetylation events, ensuring that lysine-acetylated peptides are selectively enriched from complex mixtures. By improving specificity and sensitivity at the enrichment stage, we provide high-quality samples that are ready for downstream proteomic analysis, including site identification, quantitative profiling, and pathway interpretation.

 

Protein acetylation is a key post-translational modification involved in transcriptional regulation, DNA repair, apoptosis, autophagy, metabolism, and immune signaling. Due to its typically low stoichiometry, many acetylation sites cannot be reliably detected without selective enrichment. Antibody-based enrichment of acetylated peptides has become an essential step for acetylome research, offering a powerful strategy to uncover functional roles of histone and non-histone acetylation in both eukaryotic and prokaryotic systems.

 

Services at MtoZ Biolabs

MtoZ Biolabs provides a systematic enrichment workflow designed to selectively isolate acetylated peptides from diverse biological samples. Because direct analysis often misses critical acetylation sites, enrichment substantially reduces background signals and enables reliable detection of low-abundance modifications. With our optimized protocols, enriched peptide pools are delivered in a state suitable for downstream mass spectrometry or quantitative acetylomics.

 

At the core of our Acetylated Peptide Enrichment Service is antibody-based affinity enrichment using validated pan-acetyl-lysine antibodies. Proteins are digested into peptides to expose acetylation sites, which enhances binding efficiency during enrichment. For complex projects, optional prefractionation approaches such as strong cation exchange (SCX), hydrophilic interaction chromatography (HILIC), or reverse-phase liquid chromatography (RPLC) can be integrated with antibody capture to further increase recovery and coverage. This ensures that both histone and non-histone acetylation events are comprehensively represented in the enriched samples.

 

Analysis Workflow

1. Sample Preparation

Protein extraction from cells, tissues, serum, plasma, exosomes, or microbial samples with strict quality control.

2. Protein Digestion

High-purity trypsin digestion generates peptide pools for enrichment.

3. Peptide Prefractionation (Optional)

SCX, HILIC, or RPLC fractionation can be applied to reduce sample complexity.

4. Antibody-Based Enrichment

Pan-acetyl-lysine antibodies selectively capture acetylated peptides from digested mixtures.

5. Optional Downstream Analysis

Enriched peptides are suitable for LC-MS/MS site identification, quantitative profiling, or multi-PTM studies if requested by the client.

6. Report Delivery

Results include details of enrichment efficiency and, if combined with downstream workflows, comprehensive site mapping and pathway annotations.

 

Guan, K. L. et al. Nat Protoc. 2010.

Figure 1. Schematic Diagram of Enrichment of Acetylated Peptides from Human Liver

 

Why Choose MtoZ Biolabs?

✔ Specialized Antibody-Based Enrichment

High selectivity for acetylated peptides ensures data reliability.

✔ Optional Prefractionation Strategies

SCX, HILIC, and RPLC enhance recovery and expand acetylome coverage.

✔ Seamless Integration with Downstream Analysis

Enriched peptides can be further analyzed through our proteomics platforms if required.

✔ Diverse Sample Compatibility

Applicable to cells, tissues, fluids, and microbial extracts.

✔ Expert Data Support

From enrichment validation to optional bioinformatics interpretation.

✔ Transparent Pricing

Clear one-time charge without hidden costs.

 

Sample Submission Suggestions

 

Note: Provide details on sample collection and handling. If you need further details, our technical support team is happy to assist and provide comprehensive guidance on sample submission.

 

FAQ

Q1: Can enriched peptides be used for quantitative studies?

Yes. Enriched peptides are fully compatible with quantitative workflows such as Label-Free, TMT/iTRAQ, or DIA, allowing clients to extend enrichment into quantitative acetylomics projects.

 

Q2: How do you ensure enrichment specificity?

We use validated pan-acetyl-lysine antibodies combined with optimized digestion and enrichment protocols. Optional prefractionation further reduces complexity and improves specificity.

 

Acetylated Peptide Enrichment Service at MtoZ Biolabs ensures high-specificity recovery of acetylated peptides using antibody-based enrichment strategies. By reducing background and enhancing sensitivity, we provide researchers with high-quality enriched samples ready for advanced proteomic investigations. Beyond acetylation, MtoZ Biolabs also delivers a full suite of PTM-omics services, including phosphorylation, methylation, ubiquitination, and succinylation analysis. Partner with us to advance your research with robust enrichment and PTM proteomics expertise.