Histone Acetylation Analysis Service

Based on a high-resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS) platform combined with specific peptide enrichment methods, MtoZ Biolabs' histone acetylation analysis service enables precise detection and quantitative analysis of acetylation modifications on histone lysine residues. This service can identify acetylation sites and monitor their abundance changes, with high sensitivity even for low-abundance modifications. By integrating bioinformatics tools, it provides data on modification distribution, differential comparison, and functional annotation, offering reliable support for related research.

 

Overview

Histone acetylation (Kac) is a post-translational modification on histone lysine residues, in which the attachment of an acetyl group alters chromatin conformation and affects gene transcription regulation. This modification plays an important role in regulating chromatin accessibility, maintaining gene expression activity, and controlling cellular functions, and has become a central focus of epigenetic research. Histone acetylation analysis allows precise detection and quantification of acetylation modifications at different sites, applied to studies of chromatin remodeling, cell differentiation and development, metabolic state regulation, and the discovery of potential biomarkers, providing researchers with reliable data support.

 

Fallah, M. S. et al. Front Genet. 2021.

Figure 1. Histone Acethylation and Deacetylation Mechanism.

 

Analysis Workflow

1. Histone Extraction and Digestion

Histones are extracted from cell or tissue samples and digested under optimized conditions to generate detectable peptide fragments.

 

2. Peptide Enrichment

Specific strategies are applied to enrich acetylated peptides, enhancing detection sensitivity and coverage.

 

3. Mass Spectrometry Detection

A high-resolution LC-MS/MS platform is used for accurate qualitative and quantitative analysis of acetylation sites.

 

4. Data Analysis

Bioinformatics methods are applied to provide modification distribution characteristics and functional annotation results.

 

Sample Submission Suggestions

1. Sample Type and Quantity

   

Note: Plasma should be collected using EDTA as an anticoagulant. Standard tissue or cell lysis buffers can be used during protein extraction.

 

2. Sample Transportation

Avoid repeated freeze-thaw cycles. Samples are recommended to be stored at -80°C and transported on dry ice to ensure low-temperature conditions throughout the process and prevent modification loss.

Note: For special samples or if a detailed submission plan is required, please contact MtoZ Biolabs technical staff in advance.

 

Service Advantages

1. High-Sensitivity Detection

Based on a high-resolution LC-MS/MS platform, low-abundance acetylation modifications can be accurately identified.

 

2. Strict Quality Control

Quality control is implemented throughout the entire process from sample preparation to data analysis, ensuring stable and reliable results.

 

3. One-Time-Charge

Our pricing is transparent, no hidden fees or additional costs.

 

4. Customized Solutions

Experimental workflows can be flexibly designed according to research objectives and sample types to meet diverse scientific needs.

 

Applications

1. Epigenetics Research

Through systematic detection of acetylation modifications, the mechanisms of their roles in chromatin conformation changes and transcriptional regulation can be elucidated.

 

2. Metabolic Regulation Research

Histone acetylation analysis service can be used to analyze the relationship between acetylation levels and energy metabolism pathways, nutrient supply, and cellular homeostasis regulation.

 

3. Potential Biomarker Discovery

By comparing modification patterns under different conditions, acetylation features associated with specific physiological or pathological states can be screened.

 

4. Immune Regulation Research

Histone acetylation analysis service can be used to study the regulatory functions of acetylation modifications in immune cell differentiation, activation, and inflammatory responses.

 

FAQ

Q1: Which Histone Acetylation Modifications Can This Service Analyze?

A1: Acetylation sites on multiple histone subtypes (such as H2A, H2B, H3, and H4) can be detected, including common ones such as H3K9ac, H3K27ac, and H4K16ac. Different sites are often associated with specific transcriptional activation or repression functions.

 

Q2: Can Multiple Modifications Be Analyzed Together?

A2: Yes. Acetylation often cooperates or antagonizes with modifications such as methylation, malonylation, and hydroxybutyrylation. Combined analysis helps to comprehensively elucidate modification networks and their integrated effects on gene expression and cellular functions.