Venom O-Glycan Analysis Service

Venom O-Glycan Analysis Service uses high-resolution mass spectrometry and systematic data analysis to identify, structurally characterize, and quantitatively compare O-glycans from venom proteins or peptides. It is a key approach for studying the complexity and functional diversity of venom. This analysis helps reveal glycan modification types, linkage sites, and structural isomers in venom, providing molecular-level support for studies on venom physiology, antivenom drug design, and biopharmaceutical development.

 

Relying on its advanced mass spectrometry-based proteomics platform, MtoZ Biolabs provides the Venom O-Glycan Analysis Service which enables comprehensive characterization and quantitative analysis of O-glycans from various venom sources, including snake venom, spider venom, scorpion venom, and marine toxins, offering systematic and reliable data support for research institutions and enterprises to better understand the biological significance and potential applications of venom O-glycans.

 

Analysis Workflow

1. O-glycan release and extraction

Chemical β-elimination or enzymatic digestion methods are used to specifically release O-glycans from venom proteins or glycopeptides, providing purified samples for subsequent structural analysis.

 

2. Glycan derivatization and separation

According to analytical purposes, glycans are derivatized, such as by reductive amination or labeling, to enhance detection sensitivity and structural resolution. HILIC or Porous Graphitized Carbon (PGC) chromatography is then used to separate glycan isomers.

 

3. High-resolution LC-MS/MS detection

Using high-resolution mass spectrometry systems such as Orbitrap Fusion Lumos and Q Exactive HF-X combined with multiple fragmentation strategies (HCD, CID, EThcD), structural identification and isomer recognition of O-glycans are achieved.

 

4. O-glycan structural analysis and database matching

Dedicated software such as Byonic, GlycoWorkbench, and pGlyco is used for glycan structure inference, fragment spectrum matching, and database searching to obtain glycan types, linkage sites, and composition information.

 

5. Quantification and differential analysis

Label-Free, TMT, and DIA quantification methods are supported to compare glycan abundance differences across venom sources or experimental conditions, revealing the relationship between structural characteristics and biological function.

 

6. Reporting and data visualization

Deliverables include glycan composition tables, structural diagrams, quantitative statistics, and heatmaps of glycan distribution to assist researchers in result interpretation and follow-up analysis.

 

Figure 1. Workflow for Venom O-Glycan Analysis

 

Service Advantages

1. High-resolution mass spectrometry platform

MtoZ Biolabs is equipped with multiple high-performance Orbitrap and Q-TOF mass spectrometers combined with Nano-LC systems, enabling highly sensitive detection of low-abundance O-glycans in complex venom samples.

 

2. Multiple strategies for O-glycan release and enrichment

According to venom type and sample properties, we flexibly apply chemical β-elimination, enzymatic digestion, HILIC, or PGC enrichment to maximize O-glycan recovery and structural diversity for comprehensive coverage.

 

3. Multiple quantification methods

Our venom O-Glycan analysis service supports various quantification strategies, including Label-Free, TMT, DIA, and PRM, suitable for different research stages from exploratory studies to targeted validation, providing multidimensional quantitative results for O-glycan differential analysis.

 

4. Experienced team

Our technical team has long been engaged in glycomics and venomics research, with extensive experience in distinguishing O-glycan isomers, interpreting fragment spectra, and identifying glycosylation sites, ensuring accurate and interpretable results.

 

Applications

1. Venom functional mechanism studies: revealing the regulatory role of O-glycan modifications in toxin activity, stability, and receptor binding

2. Antivenom and drug development: identifying function-related O-glycan structures to provide molecular foundations for antibody neutralization and drug targeting

3. Venom evolution and comparative studies: analyzing O-glycan differences among species or environmental conditions to explore adaptive evolution patterns

4. Structural biology and systems toxicology: supporting 3D structure analysis and functional modeling of toxins with glycan structural data

5. Biomarker screening: identifying specific O-glycan structures for species identification or venom composition tracking

 

Sample Submission Suggestions

1. Sample types: natural venom samples or recombinant venom proteins, including snake venom, spider venom, scorpion venom, insect venom, and marine toxins

2. Sample amount: recommended total protein ≥100 µg, concentration ≥1 µg/µL

Storage conditions: store at −80°C and avoid repeated freeze-thaw cycles

3. Transportation: ship on dry ice and maintain low temperature throughout transport

4. Buffer requirements: avoid high salt, high sugar, glycerol, or detergent systems that may affect MS detection

5. Additional information: provide sample source, extraction method, and research purpose so that we can design the optimal analytical plan

 

MtoZ Biolabs provides complete sample packaging and submission guidelines to ensure safe delivery and compliance with analytical standards.

 

Deliverables

1. Standardized analysis report

2. Raw data files

3. Statistical and annotation tables

4. Visual charts

5. Structural interpretation and summary

 

Contact our technical experts to obtain a customized venom O-glycan analysis solution and make MtoZ Biolabs your trusted partner in scientific research.