Venom Glycopeptides Analysis Service

Venom is a complex bioactive mixture formed during the evolution of many organisms, containing a large number of structurally unique and functionally diverse proteins and peptides. In recent years, with the advancement of mass spectrometry technology, researchers have gradually recognized the key role of glycosylation in venom biology. Glycopeptides refer to peptides carrying glycan modifications, and their structural information directly reflects the glycosylation sites and glycan types of glycoproteins. They are important research objects for revealing toxin molecular functions, structural stability, and evolutionary relationships.

 

Figure 1. Major Constituents of Snake Venom

 

Venom glycopeptides analysis systematically identifies and quantifies glycosylation modifications in venom, providing data support for understanding protein folding mechanisms, toxicity regulation, immune evasion, and interspecies variation. This type of research not only promotes the development of venom biology and structural biology but also provides new ideas for drug discovery, antivenom design, and biopharmaceutical innovation.

 

With extensive experience in mass spectrometry-based proteomics and high-resolution mass spectrometry platforms, MtoZ Biolabs has established a mature Venom Glycopeptides Analysis Service which enables systematic glycopeptide identification and quantitative analysis of various venom sources, including snake venom, spider venom, insect venom, and marine toxins. We provide high-quality and reproducible data to help researchers and enterprises deeply explore the biological significance of venom molecular modifications.

 

Services at MtoZ Biolabs

The Venom Glycopeptides Analysis Service at MtoZ Biolabs aims to comprehensively analyze the composition, modification types, and functional correlations of glycopeptides in venom. By integrating high-resolution liquid chromatography–mass spectrometry (LC-MS/MS), glycopeptide-specific enrichment strategies, and multidimensional data analysis workflows, we provide end-to-end analytical support from sample preparation to data interpretation. Main services include:

1. Comprehensive detection and identification of glycopeptides

Using high-resolution mass spectrometry based on multiple fragmentation modes such as HCD, EThcD, or CID, we systematically identify glycosylation sites and glycan structures of N- and O-linked glycopeptides in venom, helping researchers map molecular glycosylation characteristics.

 

2. Quantitative analysis of glycopeptides

Through labeling-based quantification (TMT, iTRAQ) or label-free approaches, we achieve precise comparisons of glycopeptide abundance differences, providing reliable support for functional and differential analysis.

 

3. Venom glycosylation profiling

By integrating glycomics and proteomics data, we construct glycosylation distribution maps, revealing structural and functional correlations among different venom components.

 

4. Glycopeptide structural analysis and database matching

Using bioinformatics tools for glycan structure prediction, fragment spectrum matching, and database searching, we achieve accurate annotation of glycan structures.

 

Analysis Workflow

1. Sample preparation: Venom protein extraction, quantification, and trypsin digestion; desalting and concentration when necessary

2. Glycopeptide enrichment: Specific enrichment using HILIC, lectin, or TiO₂ strategies

3. Mass spectrometry detection: High-resolution MS analysis using platforms such as Orbitrap Fusion Lumos or Q Exactive HF-X

4. Data analysis: Identification of glycopeptide sequences and glycan structures, annotation of modification sites, and relative quantification

5. Reporting: Generation of standardized analytical reports and visual charts to support interpretation and further studies

Figure 2. Workflow for Venom Glycopeptides Analysis

Service Advantages

1. High resolution and sensitivity

MtoZ Biolabs is equipped with high-performance Orbitrap Fusion Lumos and Q Exactive HF-X systems combined with Nano-LC platforms, enabling highly sensitive detection of low-abundance glycopeptides in complex venom samples.

 

2. Multi-strategy glycopeptide enrichment

According to different glycopeptide types and sample complexities, multiple enrichment methods such as HILIC, lectin, and TiO₂ are applied to preserve glycopeptide diversity and modification information, ensuring comprehensive results.

 

3. Multiple quantification strategies

Our venom glycopeptides analysis service support various glycopeptide quantification methods, including Label-Free, labeling-based, DIA (Data-Independent Acquisition), and PRM (Parallel Reaction Monitoring) targeted verification. These approaches can be flexibly selected based on sample characteristics and research goals, allowing accurate and reproducible quantitative comparisons.

 

4. One-time charge

Our pricing is transparent, with no hidden fees or additional costs.

 

Applications

✔️ Functional mechanism studies: Elucidating how glycosylation regulates toxin activity, stability, and receptor recognition

✔️ Toxin evolution and species differentiation: Comparing glycopeptide modification patterns among different venom sources to explore evolutionary divergence

✔️ Antivenom and drug development: Identifying function-related glycosylation sites to provide molecular foundations for antitoxin and drug design

✔️ Structural biology and systems toxicology: Supporting 3D toxin structure interpretation and revealing the relationship between structure and glycan modifications

 

Sample Submission Suggestions

1. Sample types: Natural samples or recombinant venom proteins such as snake venom, spider venom, scorpion venom, insect venom, or marine toxins

2. Sample quantity: Recommended total protein ≥100 µg with a concentration ≥1 µg/µL

3. Storage and transport: Store at −80°C and ship on dry ice; avoid repeated freeze-thaw cycles

4. Buffer requirements: Avoid high salt, glycerol, or detergents that may interfere with analysis

5. Additional information: Provide details on sample source, extraction method, and experimental objectives to enable customized analytical design

 

MtoZ Biolabs provides detailed sample submission guidelines and packaging recommendations to ensure sample integrity and compliance with analytical standards.

 

Deliverables

1. Comprehensive experimental report

2. High-quality raw data files

3. Data statistics and annotation tables

4. Visual analysis charts

5. Summary and biological interpretation

 

Please contact our expert team to obtain a customized venom glycopeptides analysis solution and advance your research achievements further